| Catalog number |
TE187S-100 |
| Product type | ELISA |
| Quantity |
96 Tests (12x8 breakable strip wells) |
| Standard range | 0.1-18 ng/ml |
| Analytical Sensitivity |
0.1 ng/ml |
| Sample volume | 25 µl/well |
| Species | Mouse or Rat |
| Application | The Calbiotech Mouse/Rat Testosterone ELISA is for the quantitative determination of Testosterone concentration in mouse/rat serum or plasma. |
| Principle |
The Calbiotech Mouse/Rat Testosterone ELISA is based on the principle of competitive binding between Testosterone in the test specimen and Testosterone-HRP conjugate for a constant amount of rabbit anti-Testosterone. In the incubation, goat anti-rabbit IgG-coated wells are incubated with 25ml of Testosterone standards, controls, patient samples, 100 ml Testosterone-HRP conjugate reagent and 50ml rabbit anti-Testosterone reagent at room temperature 60 minutes. During the incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody. Thus, the amount of Testosterone peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of Testosterone in the specimen increases. Unbound Testosterone peroxidase conjugate is then removed and the wells washed. Next, a solution of TMB Reagent is then added and incubated at room temperature for 15 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450nm.
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| Storage and Stability | Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing. |
| Precautions | For research use only. Not for use in diagnostic procedures. |
| References |
1. Chen, A., Bookstein, J.J., Meldrum, D.R., Diagnosis of a testosterone-secreting adrenal adenoma by selective venous catheterization. Fertil. Steril., 1991; 55: 1202-1203. 2. Granoff, A.B. and Abraham, G.E., Peripheral and adrenal venous levels of steroids in a patient with virilizing adrenal adenoma. Obstet. Gynecol., 1979; 53:111-115. 3. Bricaire, C., Raynaud, A., Benotmane, A., et al., Selective venous catheterization in the evaluation of hyperandrogenism. J. Endocrinol Invest., 1991; 14: 949-956. 4. Heinonen, P.K., Androgen production by epithelial ovarian tumours in post-menopausal women. Maturitas, 1991; 13: 117-117-122 5. Tietz, N.W. ed., Clinical Guide to Laboratory Tests, 3rd Edition, W.B. Saunders, Co., Philadelphia, 1995: 578-580. 6. USA Center for Disease Control/National Institute of Health Manual, “Biosafety in Microbiological and Biomedical Laboratories"”84 7. ICN Guide to Endocrine Testing. Diagnostic Division, ICN Biomedicals, Inc. pp. 2:33-35; 3:4-6. |
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