| Catalog number |
T3043T-100 |
| Product type | ELISA |
| Quantity |
96 Tests (12x8 breakable strip wells) |
| Standard range | 0.25-7.5 ng/ml |
| Analytical Sensitivity |
0.25 ng/ml |
| Sample volume | 25 µl/well |
| Species | Mouse or Rat |
| Application | The Calbiotech Mouse/Rat Triiodothyronine (T3) ELISA Kit is intended for the quantitative measurement of Triiodothyronine (T3) in mouse/rat serum or plasma. |
| Principle |
The Calbiotech Mouse/Rat T3 is a solid phase competitive ELISA. The samples, the working T3 enzyme conjugate, diluted in assay diluent, are added to the wells coated with anti-T3 polyclonal antibody. T3 in the patient’s serum competes with T3 enzyme conjugate for binding sites.
Unbound T3 and T3 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve is prepared relating color intensity to the concentration of the T3. |
| Storage and Stability | Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing. |
| Precautions | For research use only. Not for use in diagnostic procedures. |
| References |
1. Agharanya JC. Clinical usefulness of ELISA technique in the assessment of thyroid function. West Afr J Med 1990;9(4):258-63. 2. Baumgartner-Parzer SM; Wagner L; Reining G; Sexl V; Nowotny P; Muller M; Brunner M; Waldh¨ausl W. Increase by tri-iodothyronine of endothelin-1, fibronectin and von Willebrand factor in cultured endothelial cells. J Endocrinol 1997;154(2):231-9. 3. Maes M; Mommen K; Hendrickx D; Peeters D; D'Hondt P; Ranjan R; De Meyer F; Scharp´e S Components of biological variation, including seasonality, in blood concentrations of TSH, TT3, FT4, PRL, cortisol and testosterone in healthy volunteers. Clin Endocrinol (Oxf) 1997; 46(5):587-98. 4. Santini F; Chiovato L; Bartalena L; Lapi P; Palla R; Panichi V; Velluzzi F; Grasso L; Chopra IJ; Martino E; Pinchera A Study of serum 3,5,3'-triiodothyronine sulfate concentration in patients with systemic non-thyroidal illness. Eur J Endocrinol 1996;134(1):45-9 |
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