This product has only the Anti-Scl-70 precipitin band when tested using double immunodiffusion against rabbit thymus extract. The titer obtained will vary with the test system; however, undiluted antibody shows a strong Anti-Scl-70 precipitin band.
The specificity is verified by a precipitin line of identity with CDC-AF reference serum. This product shows no reactivity at a dilution of 1:100 when tested against purified nRNP, Ro/SS-A, La/SS-B, Sm, Mitochondrial, Ribosomal P or Jo-1 antigens in an ELISA system designed to detect all antibody classes.
0.01M Phosphate, 0.13M NaCl, 0.02% Azide
2 ml Deionized water. After reconstituting, aliquot and store frozen at -20°C.