This product has only the Anti-Jo-1 precipitin band when tested using double immunodiffusion against rabbit thymus extract. The titer obtained will vary with the test system; however, undiluted antibody shows a strong Anti-Jo-1 precipitin band.
The specificity is verified by a precipitin line of identity with the CDC-AF reference serum. This product shows no reactivity at a dilution of 1:100 when tested against purified nRNP, Ro/SS-A, La/SS-B, Scl-70, Sm, Mitochondrial, or Ribosomal P antigens in an ELISA system designed to detect IgG antibody.
Buffer:0.01M Phosphate, 0.13M NaCl, 0.02% Azide
2 ml Deionized water. After reconstituting, aliquot and store frozen at -20°C.